Molecular genetics of growth and development in Populus. III. A genetic linkage map of a hybrid poplar composed of RFLP, STS, and RAPD markers

@article{Bradshaw1994MolecularGO,
  title={Molecular genetics of growth and development in Populus. III. A genetic linkage map of a hybrid poplar composed of RFLP, STS, and RAPD markers},
  author={Harvey D. Bradshaw and Marc Villar and B. D. Watson and Kevin G. Otto and Sandy Stewart and Reinhard F. Stettler},
  journal={Theoretical and Applied Genetics},
  year={1994},
  volume={89},
  pages={167-178}
}
We have evaluated three DNA-based marker types for linkage map construction in Populus: RFLPs detected by Southern blot hybridization, STSs detected by a combination of PCR and RFLP analysis, and RAPDs. The mapping pedigree consists of three generations, with the F1 produced by interspecific hybridization between a P. trichocarpa female and a P. deltoides male. The F2 generation was made by inbreeding to the maximum degree permitted by the dioecious mating system of Populus. The applicability… CONTINUE READING
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