Molecular genetics of fungal siderophore biosynthesis and uptake: the role of siderophores in iron uptake and storage

@article{Haas2003MolecularGO,
  title={Molecular genetics of fungal siderophore biosynthesis and uptake: the role of siderophores in iron uptake and storage},
  author={Hubertus Haas},
  journal={Applied Microbiology and Biotechnology},
  year={2003},
  volume={62},
  pages={316-330}
}
  • H. Haas
  • Published 21 May 2003
  • Biology
  • Applied Microbiology and Biotechnology
To acquire iron, all species have to overcome the problems of iron insolubility and toxicity. In response to low iron availability in the environment, most fungi excrete ferric iron-specific chelators—siderophores—to mobilize this metal. Siderophore-bound iron is subsequently utilized via the reductive iron assimilatory system or uptake of the siderophore-iron complex. Furthermore, most fungi possess intracellular siderophores as iron storage compounds. Molecular analysis of siderophore… 

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References

SHOWING 1-10 OF 146 REFERENCES

Identification of members of the Aspergillus nidulans SREA regulon: genes involved in siderophore biosynthesis and utilization.

In Aspergillus nidulans, genes involved in siderophore biosynthesis and uptake are characterized by transcriptional induction under iron limitation in wild-type strain and de-repression in an sreA-deletion strain under conditions of sufficient iron supply.

Acquisition, Transport, and Storage of Iron by Pathogenic Fungi

  • D. Howard
  • Biology
    Clinical Microbiology Reviews
  • 1999
The iron-gathering mechanisms used by a pathogen in an infected host are largely unknown and can only be posited on the basis of in vitro studies at present.

SREA is involved in regulation of siderophore biosynthesis, utilization and uptake in Aspergillus nidulans

It is shown that lack of the GATA‐type transcription factor SREA in Aspergillus nidulans not only leads to derepression of siderophore biosynthesis but also to deregulation of sIDERophore‐bound iron uptake and ornithine esterase expression, suggesting the presence of an additional iron‐regulatory mechanism.

Identification of a Candida albicans Ferrichrome Transporter and Its Characterization by Expression inSaccharomyces cerevisiae *

It is demonstrated that the expression of CaArn1p is both necessary and sufficient for the nonreductive uptake of ferrichrome-iron and suggests that the transporter may be the only required component of the siderophore uptake system that is regulated by iron and Aft1p.

Characterization and Functional Analysis of the Siderophore-Iron Transporter CaArn1p in Candida albicans *

The characterization of the siderophore transporter CaArn1p in the fungal pathogen Candida albicans revealed that the transcription repressor CaTup1p repressed CaARN1 expression under iron-replete conditions via the DNA-binding protein Rfg1p.

Fungal siderophores: structures, functions and applications

There are potential applications for siderophores in medicine, reprocessing of nuclear fuel, remediation of metal-contaminated sites and the treatment of industrial waste, including enhancing pathogenicity, acting as intracellular iron storage compounds and suppressing growth of other microorganisms.

The siderophore system is essential for viability of Aspergillus nidulans: functional analysis of two genes encoding l‐ornithine N 5‐monooxygenase (sidA) and a non‐ribosomal peptide synthetase (sidC)

Partial restoration of the growth of siderophore‐deficient mutants by high concentrations of Fe2+ (but not Fe3+) indicates the presence of an additional ferrous transport system and the absence of an efficient reductive iron assmilatory system and ferricrocin biosynthesis was found to be crucial for efficient conidiation.

Iron uptake by fungi: contrasted mechanisms with internal or external reduction.

Reductive iron uptake by Candida albicans: role of copper, iron and the TUP1 regulator.

The opposite regulation of two iron permease genes and the role of TUP1 indicate that the process of iron acquisition by C. albicans may be more complex and potentially more adaptable than by S. cerevisiae.

The Siderophore Iron Transporter of Candida albicans (Sit1p/Arn1p) Mediates Uptake of Ferrichrome-Type Siderophores and Is Required for Epithelial Invasion

The results suggest that siderophore uptake by Sit1p/Arn1p is required in a specific process of C. albicans infection, namely epithelial invasion and penetration, while in the blood or within organs other sources of iron, including heme, may be used.
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