Molecular genetic studies of cellular senescence

  title={Molecular genetic studies of cellular senescence},
  author={Olivia Pereira-smith and Yi Ning},
  journal={Experimental Gerontology},

Accelerated aging of dermal fibroblast-like cells from the senescence-accelerated mouse (SAM): acceleration of changes in DNA ploidy associated with in vitro cellular aging.

The results suggest that the cell lines from SAMP11 mice might have higher susceptibility to factors that cause polyploidization, including oxidative stress.

Induction of erythroid gene expression by microcell fusion.

It is concluded that microcells from erythroid cells contain all the information necessary to induce expression of multiple eryhroid genes and analysis of the components of the microcells responsible for this new gene induction may allow the characterization of cellular factors responsible for erystroid-specific gene expression.

Emerging technologies: trendy RNA tools for aging research.

Aging is an inevitable biological phenomenon. Attempts to understand its mechanisms and, consequently, to therapeutically decelerate or even reverse the process are limited by its daunting

Robustness during Aging—Molecular Biological and Physiological Aspects

Here, various properties of robust behavior during human and animal aging are presented, including physiological and molecular biological features, such as the hematocrit, body temperature, immunity against infectious diseases and others.

Unregulated inflammation shortens human functional longevity

  • S. Brod
  • Biology, Medicine
    Inflammation Research
  • 2000
The premature hyperIMmunity of autoimmunity, the local "brain inflammatory response" to Aβ protein in AD, and the immune response to fatty changes in vessels in atherosclerosis all signal the critical importance of unregulated systemic inflammation to common neurological and cardiovascular disease that shortens the nominal longevity of humans.


This review is to study the exceptional herbs that may act as natural anti-ageing which can be proven to be effective in long run to counter traditional conceptualization of aging as a time of disease.

The versatile stress protein mortalin as a chaperone therapeutic agent.

How the current tools used in studying mortalin could be creatively applied in a clinical setting to manage stress and to treat various chaperone-based maladies or "chaperonopathies" is discussed.



Hybrids from fusion of normal human T lymphocytes with immortal human cells exhibit limited life span

F fused normal human T lymphocytes with an immortal human cell line to determine whether they could restore the senescent, nondividing phenotype in hybrids, as do normal human fibroblasts, and provides evidence that cellular senescence in T lymphocyte occurs via genetic mechanisms.

Immortal phenotype of the HeLa variant D98 is recessive in hybrids formed with normal human fibroblasts

The results indicate that the majority of D98 x HDF hybrid clones exhibit a clear‐cut finite proliferative lifespan phenotype, which leads us to conclude that D98 cells do not express a dominant immortalizing gene.

Reversible cellular senescence: implications for immortalization of normal human diploid fibroblasts

IMR-90 normal human diploid fibroblasts, transfected with a steroid inducible mouse mammary tumor virus-driven simian virus 40 T antigen, were carried through crisis to yield an immortal cell line.

Evidence for the recessive nature of cellular immortality.

It was found that at least two separate events in the normal cell genome can result in immortality, and in fusions involving certain immortal parent cells, these events can be complemented to result in hybrids with finite division capacity.

Replicative senescence: the human fibroblast comes of age.

Identification of participating genes and clarification of their mechanisms of action will help to elucidate the universal cellular decline of biological aging and an important obverse manifestation, the rare escape of cells from senescence leading to immortalization and oncogenesis.

Expression of SV40 T antigen in finite life-span hybrids of normal and SV40-transformed fibroblasts

Even though viral DNA was present and being expressed in all hybrid clones, the senescent phenotype was dominant in these hybrids, and the change to an indefinite life-span was a rare event.

Functional simian virus 40 T antigen is expressed in hybrid cells having finite proliferative potential

Fusions with various other immortal cells yielded hybrids that had limited division potential, and T antigen expressed in limited-division hybrids was functional for the induction of cellular DNA synthesis.