Molecular genetic characterization of the Escherichia coli gntT gene of GntI, the main system for gluconate metabolism.

@article{Porco1997MolecularGC,
  title={Molecular genetic characterization of the Escherichia coli gntT gene of GntI, the main system for gluconate metabolism.},
  author={A Porco and N. Peekhaus and Christoph Bausch and Suxiong Tong and Tom{\'a}s Ist{\'u}riz and Tyrrell Conway},
  journal={Journal of bacteriology},
  year={1997},
  volume={179 5},
  pages={1584-90}
}
The Escherichia coli gntT gene was subcloned from the Kohara library, and its expression was characterized. The cloned gntT gene genetically complemented mutant E. coli strains with defects in gluconate transport and directed the formation of a high-affinity gluconate transporter with a measured apparent Km of 6 microM for gluconate. Primer extension analysis indicated two transcriptional start sites for gntT, which are separated by 66 bp and which give rise to what appears on a Northern blot… CONTINUE READING

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