Molecular genetic basis of ribotyping.

  title={Molecular genetic basis of ribotyping.},
  author={Val{\'e}rie Bouchet and Heather Huot and Richard Z. Goldstein},
  journal={Clinical microbiology reviews},
  volume={21 2},
  pages={262-73, table of contents}
Nearly 2,000 ribotyping-based studies exist, ranging from epidemiology to phylogeny and taxonomy. None precisely reveals the molecular genetic basis, with many incorrectly attributing detected polymorphisms to rRNA gene sequences. Based on in silico genomics, we demonstrate that ribotype polymorphisms result from sequence variability in neutral housekeeping genes flanking rRNA operons, with rRNA gene sequences serving solely as conserved, flank-linked tags. We also reveal that from such an… CONTINUE READING