Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens

  title={Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens},
  author={Bruno Canard and Thierry Garnier and Brigitte Saint-Joanis and Stewart T Cole},
  journal={Molecular and General Genetics MGG},
A recombinant lambda phage was identified in a Clostridium perfringens genomic library by means of its ability to hydrolyse the fluorescent substrate 4-methyl-umbelliferyl-β-d-glucosaminide, isolated and shown to encode an endo-β-N-acetylglucosaminidase. This enzyme, NagH, is also known as hyaluronidase, or Mu toxin, a putative virulence factor which is likely to act on connective tissue during gas gangrene. Nucleotide sequence analysis allowed the primary structure to be deduced and showed… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 24 extracted citations


Publications referenced by this paper.
Showing 1-10 of 41 references

Molecular genetic studies of UV-inducible Bacteriocin production in Clostridium perfringens

  • ST Cole, T Garnier
  • Sebald M (ed) Genetics and Molecular Biology of…
  • 1993

Gene cloning and organisation of the alpha - toxin of Clostridium perfringens

  • RW Titball, H Yeoman, Hunter SEC
  • 1992

, piP 404 , from Clostridium perfringens

  • KS Gilmore, Russel RRB, J Ferretti
  • 1990

Analysis of the Streptococcus downei gtfs gene which specifies a glucosyltransferase that synthesizes soluble glucans

  • KS Gilmore, RRB Russel, J Ferretti
  • Infect Immun
  • 1990
1 Excerpt

Similar Papers

Loading similar papers…