Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens

@article{Canard1994MolecularGA,
  title={Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens},
  author={Bruno Canard and Thierry Garnier and Brigitte Saint-Joanis and Stewart T Cole},
  journal={Molecular and General Genetics MGG},
  year={1994},
  volume={243},
  pages={215-224}
}
A recombinant lambda phage was identified in a Clostridium perfringens genomic library by means of its ability to hydrolyse the fluorescent substrate 4-methyl-umbelliferyl-β-d-glucosaminide, isolated and shown to encode an endo-β-N-acetylglucosaminidase. This enzyme, NagH, is also known as hyaluronidase, or Mu toxin, a putative virulence factor which is likely to act on connective tissue during gas gangrene. Nucleotide sequence analysis allowed the primary structure to be deduced and showed… CONTINUE READING

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