Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks.

@article{Boerries2013MolecularFO,
  title={Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks.},
  author={Melanie Boerries and Florian Grahammer and Sven Eiselein and Moritz Buck and Charlotte Meyer and Markus Goedel and Wibke Bechtel and Stefan Zschiedrich and Dietmar Pfeifer and Denis Lalo{\"e} and Christelle Arrondel and Sara Gonçalves and Marcus Krueger and Scott J. Harvey and Hauke Busch and Joern Dengjel and Tobias B Huber},
  journal={Kidney international},
  year={2013},
  volume={83 6},
  pages={1052-64}
}
A thorough characterization of the transcriptome and proteome of endogenous podocytes has been hampered by low cell yields during isolation. Here we describe a double fluorescent reporter mouse model combined with an optimized bead perfusion protocol and efficient single cell dissociation to yield more than 500,000 podocytes per mouse allowing for global, unbiased downstream applications. Combining mRNA and miRNA transcriptional profiling with quantitative proteomic analyses revealed programs… CONTINUE READING
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