Molecular features determining different partitioning patterns of papain and bromelain in aqueous two-phase systems.

@article{Rocha2013MolecularFD,
  title={Molecular features determining different partitioning patterns of papain and bromelain in aqueous two-phase systems.},
  author={Mar{\'i}a Victoria Rocha and Bibiana Beatriz Nerli},
  journal={International journal of biological macromolecules},
  year={2013},
  volume={61},
  pages={
          204-11
        }
}
The partitioning patterns of papain (PAP) and bromelain (BR), two well-known cysteine-proteases, in polyethyleneglycol/sodium citrate aqueous two-phase systems (ATPSs) were determined. Polyethyleneglycols of different molecular weight (600, 1000, 2000, 4600 and 8000) were assayed. Thermodynamic characterization of partitioning process, spectroscopy measurements and computational calculations of protein surface properties were also carried out in order to explain their differential partitioning… CONTINUE READING
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