Molecular farming - The slope of enlightenment.

  title={Molecular farming - The slope of enlightenment.},
  author={Rainer Fischer and Johannes Felix Buyel},
  journal={Biotechnology advances},

Targeted genome editing of plants and plant cells for biomanufacturing

The effects of metabolic optimization, adaptation of the endomembrane system, modified glycosylation profiles, programmable growth and senescence, protease inactivation, and the expression of enzymes that promote biodegradation are discussed.

Specialized Metabolites and Valuable Molecules in Crop and Medicinal Plants: The Evolution of Their Use and Strategies for Their Production

An overview of the novel studies recently published regarding plant biotechnological, genetics and metabolic insights paves the path to novel applications of the so-called molecular farming.

Genetic Containment for Molecular Farming

This review summarized some of the production systems available for molecular farming purposes and options to implement or improve plant containment.

Rational domestication of a plant-based recombinant expression system expands its biosynthetic range

The rational domestication of Nicotiana benthamiana within two generations is demonstrated to endow this plant molecular farming host with an expanded repertoire of peptide sequence space, and molecules including an insecticidal peptide, a prostate cancer therapeutic lead and an orally active analgesic are demonstrated.

Flexible approaches are required for successful production of recombinant proteins in plants

In this mini-review, the approaches that are needed for the production of functional recombinant proteins such as vaccines, antibodies, enzymes for medical, agricultural, and industrial applications are discussed.

Plant-Derived Human Vaccines: Recent Developments

The field has subsequently advanced with improved expression techniques including a shift from using transgenic to transient expression of antigens, refinement of purification protocols, a deeper understanding of the biological processes and a wealth of evidence of immunogenicity and efficacy of plant-produced vaccine candidates, all contributing to the successful practice of biopharming or plant molecular farming.

Plant Molecular Farming, a Tool for Functional Food Production.

An updated overview of PMF for functional food development is presented, including the progress, problem, and strategy, and it is speculated how to use the PMF strategy to produce functional foods, especially with four major staple food crops.


The purpose of biotechnology in pharmaceutical industry, particularly pharmaceutical biotechnology along with its prospects and challenges are described.



Molecular Farming in Plants: The Long Road to the Market

Recombinant proteins can be produced on a commercial scale using a diverse array of host systems based on microbes, animals, and plants. Commercially established processes have resolved to a small

Optimizing the yield of recombinant pharmaceutical proteins in plants.

The diverse approaches that have been used to increase the productivity of plant-based platforms for the production of recombinant pharmaceutical proteins are discussed and future opportunities to maximize the potential of plants and increase their competitiveness outside niche markets are considered.

Critical Analysis of the Commercial Potential of Plants for the Production of Recombinant Proteins

The strengths of plant expression systems for specific applications are discussed, but the bottlenecks that must be overcome before plants can compete with conventional systems are addressed, enabling the future commercial utilization of plants for the production of valuable proteins.

Process Development Strategies in Plant Molecular Farming.

  • J. Buyel
  • Biology
    Current pharmaceutical biotechnology
  • 2015
Recent improvements in clarification and integrated process methods will have a strong impact on the economic feasibility of production, especially if supported by and combined with process analytical technology as part of the quality-by-design initiative.

Plant Molecular Farming – Integration and Exploitation of Side Streams to Achieve Sustainable Biomanufacturing

The single-use properties of plants, the sustainability of the production platform, and the commercial potential of different biomass side streams are discussed, finding that incorporating these side streams through rational process integration has the potential to more than double the revenue that can be achieved using plant-based production systems.

Molecular farming of pharmaceutical proteins

The promise of using transgenic plants as bioreactors for the molecular farming of recombinant therapeutics, including vaccines, diagnostics, such as recombinant antibodies, plasma proteins, cytokines and growth factors, is demonstrated.

Plant Molecular Farming: Much More than Medicines.

This review explores the areas where plant-based manufacturing can make the greatest impact, focusing on commercialized products such as antibodies, enzymes, and growth factors that are used as research-grade or diagnostic reagents, cosmetic ingredients, and biosensors or biocatalysts.

Edible plants for oral delivery of biopharmaceuticals.

The unique features that make plants ideal as platforms for the oral delivery of protein-based therapeutics are considered and recent developments in the production of plant derived biopharmaceuticals for oral administration are described.