Molecular evidence for a positive role of Spt4 in transcription elongation.

@article{Rondn2003MolecularEF,
  title={Molecular evidence for a positive role of Spt4 in transcription elongation.},
  author={Ana G. Rond{\'o}n and Mar{\'i}a Luisa Garc{\'i}a-Rubio and Sergio Gonz{\'a}lez-Barrera and Andr{\'e}s Aguilera},
  journal={The EMBO journal},
  year={2003},
  volume={22 3},
  pages={612-20}
}
We have previously shown that yeast mutants of the THO complex have a defect in gene expression, observed as an impairment of lacZ transcription. Here we analyze the ability of mutants of different transcription elongation factors to transcribe lacZ. We found that spt4Delta, like THO mutants, impaired transcription of lacZ and of long and GC-rich DNA sequences fused to the GAL1 promoter. Using a newly developed in vitro transcription elongation assay, we show that Spt4 is required in elongation… CONTINUE READING

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