Molecular epidemiology of enterovirus outbreaks in Canada during 1991-1992: identification of echovirus 30 and coxsackievirus B1 strains by amplicon sequencing.

@article{Drebot1994MolecularEO,
  title={Molecular epidemiology of enterovirus outbreaks in Canada during 1991-1992: identification of echovirus 30 and coxsackievirus B1 strains by amplicon sequencing.},
  author={Michael A. Drebot and Christopher Y. Nguan and J J Campbell and Sae Hwan Lee and Kevin R. Forward},
  journal={Journal of medical virology},
  year={1994},
  volume={44 4},
  pages={340-7}
}
The relatedness of enteroviral isolates associated with two recent outbreaks in Canada was assessed using direct sequencing of amplicons derived from a large portion of the 5' nontranslated region (NTR) of the viral genome. The amplicons of 60 echovirus 30 isolates originating from seven different provinces in 1991 were found to share 99% or greater sequence identity. Recent coxsackievirus B1 isolates characterised in the same manner were identical to each other. When the 5' NTR sequence of… CONTINUE READING