Molecular epidemiology of airway colonisation by Aspergillus fumigatus in cystic fibrosis patients.

@article{Cimon2001MolecularEO,
  title={Molecular epidemiology of airway colonisation by Aspergillus fumigatus in cystic fibrosis patients.},
  author={Bernard Cimon and Françoise Symoens and Rachid Zouhair and Dominique Chabasse and Nicole Nolard and A. Defontaine and J. Ph. Bouchara},
  journal={Journal of medical microbiology},
  year={2001},
  volume={50 4},
  pages={367-74}
}
A total of 109 sequential and multiple Aspergillus fumigatus isolates corresponding to 41 samples from seven cystic fibrosis (CF) patients was typed by random amplification of polymorphic DNA (RAPD) with the primer NS3 from the fungal ribosomal gene 18S subunit, and by sequence-specific DNA primer (SSDP) analysis. RAPD typing of the isolates revealed 10 different genotypes, whereas nine genotypes were identified by SSDP. Combination of the two typing methods permitted the differentiation of 25… CONTINUE READING

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