Molecular engineering approaches to peptide, polyketide and other antibiotics

@article{Baltz2006MolecularEA,
  title={Molecular engineering approaches to peptide, polyketide and other antibiotics},
  author={Richard H. Baltz},
  journal={Nature Biotechnology},
  year={2006},
  volume={24},
  pages={1533-1540}
}
  • R. H. Baltz
  • Published 1 December 2006
  • Biology, Medicine
  • Nature Biotechnology
Molecular engineering approaches to producing new antibiotics have been in development for about 25 years. Advances in cloning and analysis of antibiotic gene clusters, engineering biosynthetic pathways in Escherichia coli, transfer of engineered pathways from E. coli into Streptomyces expression hosts, and stable maintenance and expression of cloned genes have streamlined the process in recent years. Advances in understanding mechanisms and substrate specificities during assembly by polyketide… 
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Sequential targeted gene manipulation involved in polyketide biosynthetic reguation synergistically provided an efficient and rational strategy for Streptomyces strain improvement.
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TLDR
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TLDR
The elucidation of the molecular machinery of antibiotic biosynthesis enabled technologies that can be used to optimize the secondary metabolites by genetic engineering are highlighted and some strategies are highlighted which allow the modification of secondary metabolites in ways that were hard to achieve using only synthetic chemistry approaches.
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