Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA.

@article{Heilig2002MolecularDO,
  title={Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA.},
  author={Hans G. H. J. Heilig and Erwin G. Zoetendal and Elaine E. Vaughan and Philippe Marteau and Antoon D. L. Akkermans and Willem M de Vos},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 1},
  pages={114-23}
}
A Lactobacillus group-specific PCR primer, S-G-Lab-0677-a-A-17, was developed to selectively amplify 16S ribosomal DNA (rDNA) from lactobacilli and related lactic acid bacteria, including members of the genera Leuconostoc, Pediococcus, and WEISSELLA: Amplicons generated by PCR from a variety of gastrointestinal (GI) tract samples, including those originating from feces and cecum, resulted predominantly in Lactobacillus-like sequences, of which ca. 28% were most similar to the 16S rDNA of… CONTINUE READING
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