Molecular distances from dipolar coupled spin-labels: the global analysis of multifrequency continuous wave electron paramagnetic resonance data.

@article{Hustedt1997MolecularDF,
  title={Molecular distances from dipolar coupled spin-labels: the global analysis of multifrequency continuous wave electron paramagnetic resonance data.},
  author={Eric J. Hustedt and Alex I Smirnov and Charles F. Laub and Charles E. Cobb and Albert H. Beth},
  journal={Biophysical journal},
  year={1997},
  volume={72 4},
  pages={1861-77}
}
For immobilized nitroxide spin-labels with a well-defined interprobe geometry, resolved dipolar splittings can be observed in continuous wave electron paramagnetic resonance (CW-EPR) spectra for interelectron distances as large as 30 A using perdeuterated probes. In this work, algorithms are developed for calculating CW-EPR spectra of immobilized, dipolar coupled nitroxides, and then used to define the limits of sensitivity to the interelectron distance as a function of geometry and microwave… CONTINUE READING

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