Molecular differential expression of voltage-gated sodium channel α and β subunit mRNAs in five different mammalian cell lines.

@article{Baroni2011MolecularDE,
  title={Molecular differential expression of voltage-gated sodium channel α and β subunit mRNAs in five different mammalian cell lines.},
  author={Debora Baroni and Oscar Mor{\'a}n},
  journal={Journal of bioenergetics and biomembranes},
  year={2011},
  volume={43 6},
  pages={729-38}
}
Voltage-gated sodium channels are composed of one α subunit and one or more auxiliary β subunits. A standard reverse transcription-polymerase chain reaction assay was used to detect the mRNAs encoding for seven α subunits (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.5, Nav1.6, Nav1.9) and for the two non-covalently linked β1 and β3 auxiliary subunits in five different cell lines from rat, mouse and human origin. A semi-quantitavive RT-PCR analysis allowed to evaluate in each cell line, the relative… CONTINUE READING