Molecular diagnosis of subcutaneous Pythium insidiosum infection by use of PCR screening and DNA sequencing.

@article{Salipante2012MolecularDO,
  title={Molecular diagnosis of subcutaneous Pythium insidiosum infection by use of PCR screening and DNA sequencing.},
  author={Stephen J. Salipante and Daniel R. Hoogestraat and Dhruba J. Sengupta and Donald K Murphey and Kyriacos Panayides and Emma Hamilton and Irene Casta{\~n}eda-S{\'a}nchez and Jason Kennedy and Peter W Monsaas and Leonel Mendoza and Karen G Stephens and James J. Dunn and Brad T. Cookson},
  journal={Journal of clinical microbiology},
  year={2012},
  volume={50 4},
  pages={1480-3}
}
Pythium insidiosum is an emerging human pathogen classified among brown algae and diatoms that can cause significant morbidity and mortality in otherwise healthy individuals. Here we describe a pediatric patient with pythiosis acquired in the southern United States, diagnosed by molecular screening and DNA sequencing of internal transcribed spacer region 1.