Molecular diagnosis and epidemiology of fungal infections.

  title={Molecular diagnosis and epidemiology of fungal infections.},
  author={E. Rei\ss and Kiyoshi Tanaka and G Bruker and Val{\'e}rie Chazalet and David Coleman and J. P. Debeaupuis and Ryo Hanazawa and Jean Paul Latg{\'e} and J Lortholary and K. Makimura and Christine J Morrison and Somay Yamagata Murayama and Shiro Naoe and Sophie Paris and Jacqueline Sarfati and Kazutoshi Shibuya and Derek Sullivan and Katsuhisa Uchida and Hideyo Yamaguchi},
  journal={Medical mycology},
  volume={36 Suppl 1},
A variety of methods are utilized for DNA strain subtyping of Candida spp. because no 'gold standard' exists. Random amplified polymorphic DNA (RAPD) or restriction enzyme analysis (REA) are useful to determine the source of an outbreak, but more reproducible and discriminatory methods such as Southern hybridization and pulsed field gel electrophoresis (PFGE) may be required. When applied to some nosocomial Candida infections, multiple strains and species have been identified. Microevolution of… CONTINUE READING


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