Molecular detection of feline calicivirus in clinical samples: A study comparing its detection by RT-qPCR directly from swabs and after virus isolation.

@article{Meli2018MolecularDO,
  title={Molecular detection of feline calicivirus in clinical samples: A study comparing its detection by RT-qPCR directly from swabs and after virus isolation.},
  author={Marina L Meli and Alice Berger and Barbara Willi and Andrea M. Spiri and Barbara Riond and Regina Hofmann-Lehmann},
  journal={Journal of virological methods},
  year={2018},
  volume={251},
  pages={
          54-60
        }
}
Feline caliciviruses (FCVs) are non-enveloped RNA viruses that exhibit high genetic variation. Two reverse transcription quantitative polymerase chain reaction (RT-qPCR) FCV assays (S1 and S2) were evaluated using samples from 300 field cats. The direct detection of FCV in swabs and after propagation in cell culture, as well as the influence of storage conditions, was assessed. FCV-RNA detectability on dry swabs was similar after storage at either 4°C or -20°C, but viral burdens were maintained… CONTINUE READING
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