Corpus ID: 56334377

Molecular detection of Clostridium perfringens type D alpha and epsilon toxin genes from various tissues in lambs.

@article{Nasir2013MolecularDO,
  title={Molecular detection of Clostridium perfringens type D alpha and epsilon toxin genes from various tissues in lambs.},
  author={A. Nasir and M. Younus and Muti-ur-Rehman and M. Latif and A. Rashid and R. Ahmad and M. Abbas},
  journal={Pakistan Veterinary Journal},
  year={2013},
  volume={33},
  pages={492-495}
}
Received: Revised: Accepted: December 13, 2012 May 25, 2013 June 05, 2013 Different tissues including duodenal scrapings, liver, lungs and kidneys of each infected (n=6) and control (n=4) lambs were subjected to PCR for the identification of alpha and epsilon toxin genes of Clostridium perfringens type D. Alpha and epsilon toxin genes were amplified at annealing temperature 52.2 and 50.2C with amplicon size about 247bp and 665bp, respectively. A high percentage of alpha toxin genes of C… Expand

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References

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PCR detection of Clostridium perfringens type D in formalin‐fixed, paraffin‐embedded tissues of goats and sheep
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