Molecular detection and identification of Synergistes phylotypes in primary endodontic infections.

@article{Siqueira2007MolecularDA,
  title={Molecular detection and identification of Synergistes phylotypes in primary endodontic infections.},
  author={Jos{\'e} F Siqueira and Isabela das Neves R{\^o}ças},
  journal={Oral diseases},
  year={2007},
  volume={13 4},
  pages={398-401}
}
AIM Uncultivated phylotypes from the Synergistes group have recently emerged as suspected endodontic pathogens. This study aimed to investigate the presence and identity of these bacteria in primary endodontic infections using a 16S rRNA gene-based group-specific heminested polymerase chain reaction (PCR) protocol. MATERIALS AND METHODS Genomic DNA was isolated directly from clinical samples and used as templates for PCR. Amplicons from positive specimens were sequenced and phylogenetically… CONTINUE READING

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