Molecular crowding enhances native structure and stability of alpha/beta protein flavodoxin.

@article{Stagg2007MolecularCE,
  title={Molecular crowding enhances native structure and stability of alpha/beta protein flavodoxin.},
  author={Loren J. Stagg and Shao-Qing Zhang and Margaret S. Cheung and Pernilla Wittung-Stafshede},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2007},
  volume={104 48},
  pages={18976-81}
}
To investigate the consequences of macromolecular crowding on the behavior of a globular protein, we performed a combined experimental and computational study on the 148-residue single-domain alpha/beta protein, Desulfovibrio desulfuricans apoflavodoxin. In vitro thermal unfolding experiments, as well as assessment of native and denatured structures, were probed by using far-UV CD in the presence of various amounts of Ficoll 70, an inert spherical crowding agent. Ficoll 70 has a concentration… CONTINUE READING

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