Molecular coproscopy: dung and diet of the extinct ground sloth Nothrotheriops shastensis.

  title={Molecular coproscopy: dung and diet of the extinct ground sloth Nothrotheriops shastensis.},
  author={Hendrik N. Poinar and Michael Hofreiter and W. Geoffrey Spaulding and P. S. Martin and B. Artur Stankiewicz and Helen A. Bland and Richard P. Evershed and G{\"o}ran Possnert and Svante P{\"a}{\"a}bo},
  volume={281 5375},
DNA from excrements can be amplified by means of the polymerase chain reaction. [] Key Result A chemical agent, N-phenacylthiazolium bromide, that cleaves such cross-links made it possible to amplify DNA sequences. Analyses of these DNA sequences showed that the coprolite is derived from an extinct sloth, presumably the Shasta ground sloth Nothrotheriops shastensis. Plant DNA sequences from seven groups of plants were identified in the coprolite. The plant assemblage that formed part of the sloth's diet…

A molecular analysis of ground sloth diet through the last glaciation

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Nuclear Gene Sequences from a Late Pleistocene Sloth Coprolite

First coprolite evidence for the diet of Anomalopteryx didiformis, an extinct forest ratite from New Zealand

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Coprolites are fossil scats and provide indirect witness of the activity of past animals of a given area, whether or not fossil bones of these animals are present in the site. The shape, size,

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Metagenomic analysis of coprolites from three Late Pleistocene megaherbivores from the Southwestern United States

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The following method, which is a modification of a protocol published by Boom et a/.

Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.

  • S. Pääbo
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1989
The polymerase chain reaction can be used to amplify and study short mitochondrial DNA sequences that are of anthropological and evolutionary significance and opens up the prospect of performing diachronical studies of molecular evolutionary genetics.

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The retrieval of ancient human DNA sequences.

These results show that more experimental work than is often applied is necessary to ensure that DNA sequences amplified from ancient human remains are authentic and quantitation of the numbers of amplifiable molecules is a useful tool to determine the role of contaminating contemporary molecules and PCR errors in amplifications from ancient DNA.

Death of American Ground Sloths

Dates from other caves in the arid Southwest indicate that the Shasta ground sloth disappeared at very soon after the time of Clovis big game hunters, in accord with the model of explosive overkill.

The Maillard reaction in foods.

The classical methods used to inhibit browning are reviewed and then the recent work on acyclic formation rate of sugars and cysteine inhibition is focused on.

Modification of DNA by reducing sugars: a possible mechanism for nucleic acid aging and age-related dysfunction in gene expression.

It is concluded that glucose, Glc-6-P, and possibly other sugars can react with DNA to produce significant structural and biological alterations.