Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses

@article{Doszpoly2008MolecularCO,
  title={Molecular confirmation of a new herpesvirus from catfish (Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses},
  author={Andor Doszpoly and Endre R. Kov{\'a}cs and Giuseppe Bovo and Scott E. Lapatra and Bal{\'a}zs Harrach and M{\'a}ria Benkő},
  journal={Archives of Virology},
  year={2008},
  volume={153},
  pages={2123-2127}
}
A PCR method with consensus degenerate primers was developed for the detection of herpesviruses (HVs) of anamnia. Compared to previously published PCRs, targeting the DNA polymerase gene of fish HVs, the size of PCR products was more than tripled. Although broad applicability of the method could not be proven, approximately 1,600-bp fragments from HVs of white sturgeon (Acipenser transmontanus) and black bullhead (Ameiurus melas) were obtained and sequenced. Phylogenetic tree reconstructions… CONTINUE READING
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