• Biology, Medicine
  • Published in
    The Journal of biological…
    1993

Molecular cloning of two DNA-binding proteins of maize that are structurally different but interact with the same sequence motif.

@article{Yanagisawa1993MolecularCO,
  title={Molecular cloning of two DNA-binding proteins of maize that are structurally different but interact with the same sequence motif.},
  author={Shuichi Yanagisawa and Katsura Izui},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 21},
  pages={
          16028-36
        }
}
Nuclear extracts from maize leaves have been shown previously to contain a factor, MNF1, that interacts with both the cauliflower mosaic virus 35S promoter and the promoter of the maize gene for phosphoenolpyruvate carboxylase, which is involved in C4 photosynthesis. We have isolated two cDNA clones encoding proteins (MNB1a and MNB1b), that bind to an MNF1-binding site in a sequence-specific manner, by screening of a maize cDNA expression library with synthetic oligonucleotides. Using various… CONTINUE READING

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