Molecular cloning of the wild-type phoM operon in Escherichia coli K-12.

@article{Wanner1988MolecularCO,
  title={Molecular cloning of the wild-type phoM operon in Escherichia coli K-12.},
  author={Barry L. Wanner and Manfred Wilmes and Eric J. Hunter},
  journal={Journal of bacteriology},
  year={1988},
  volume={170 1},
  pages={
          279-88
        }
}
A metastable bacterial alkaline phosphatase (Bap) phenotype is seen in phoR mutants, which alternately express a Bap-constitutive or -negative phenotype. The alteration is affected by mutations in the phoM region near 0 min. By molecular cloning of the wild-type phoM operon onto a multicopy plasmid and recombining onto the plasmid the pho-510 mutation that abolishes variation, the phoM operon, rather than some nearby gene, was shown to control variation. Complementation tests indicated that the… CONTINUE READING