Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.

@article{Kato1990MolecularCO,
  title={Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.},
  author={Nobuyuki Kato and Makoto Hijikata and Yuko Ootsuyama and Masanori Nakagawa and Showgo Ohkoshi and Takashi Sugimura and Kunitada Shimotohno},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 24},
  pages={
          9524-8
        }
}
  • N. Kato, M. Hijikata, K. Shimotohno
  • Published 1 December 1990
  • Biology
  • Proceedings of the National Academy of Sciences of the United States of America
The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese patients with chronic hepatitis. HCV-J genome contains a long open reading frame that can encode a sequence of 3010 amino acid residues. Comparison of HCV-J with the American isolate of HCV showed 22.6% difference in nucleotide sequence and 15.1% difference in amino acid sequence. Thus HCV-J and the… 
cDNA clones of Japanese hepatitis C virus genomes derived from a single patient show sequence heterogeneity.
TLDR
Twelve cDNA clones of Japanese hepatitis C virus (HCV) have been isolated from liver tissue of a single non-A, non-B hepatitis patient, suggesting that repeated infections of different HCVs may have occurred in the patient.
Genomic characterization and mutation rate of hepatitis C virus isolated from a patient who contracted hepatitis during an epidemic of non-A, non-B hepatitis in Japan.
TLDR
It is suggested that the outbreak in Shimizu city was caused by a strain of HCV closely related to the Japanese-like subgroup of isolates, which is suggested to have been caused by the strain derived from the serum of a chimpanzee inoculated with the same serum 9 years previously.
Derivation of a Rational Nomenclature for Hepatitis C Virus by Phylogenetic Analysis of the NS-5 Region
TLDR
A new nomenclature for HCV variants is proposed in this communication that reflects the two-tiered nature of sequence differences between different viral isolates.
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