Molecular cloning of the human Goodpasture antigen demonstrates it to be the alpha 3 chain of type IV collagen.

@article{Turner1992MolecularCO,
  title={Molecular cloning of the human Goodpasture antigen demonstrates it to be the alpha 3 chain of type IV collagen.},
  author={Nicholas C. Turner and P. J. Mason and Robin Brown and M H Fox and Sue Povey and Adam C Rees and Charles Dickson Pusey},
  journal={The Journal of clinical investigation},
  year={1992},
  volume={89 2},
  pages={592-601}
}
To characterize the autoantigen of Goodpasture's (anti-glomerular basement membrane) disease, a molecule of 26-kD reactive with autoantibodies from patients' sera was purified from collagenase digests of sheep glomerular basement membrane. Short internal amino acid sequences were obtained after tryptic or cyanogen bromide cleavage, and used to deduce redundant oligonucleotides for use in the polymerase chain reaction on cDNA derived from sheep renal cortex. Molecules of 175 bp were amplified… CONTINUE READING