Molecular cloning of the gene for the E1 alpha subunit of the pyruvate dehydrogenase complex from Saccharomyces cerevisiae.

Abstract

The E1 alpha and E1 beta subunits of the pyruvate dehydrogenase complex from the yeast Saccharomyces cerevisiae were purified. Antibodies raised against these subunits were used to clone the corresponding genes from a genomic yeast DNA library in the expression vector lambda gt11. The gene encoding the E1 alpha subunit was unique and localized on a 1.7-kb… (More)

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@article{Steensma1990MolecularCO, title={Molecular cloning of the gene for the E1 alpha subunit of the pyruvate dehydrogenase complex from Saccharomyces cerevisiae.}, author={H. Y. de Steensma and Lennart Holterman and Irene Dekker and Christiaan van Sluis and Theresa Wenzel}, journal={European journal of biochemistry}, year={1990}, volume={191 3}, pages={769-74} }