Molecular cloning of the Escherichia coli O75X adhesin.

  title={Molecular cloning of the Escherichia coli O75X adhesin.},
  author={Bogdan J. Nowicki and James P. Barrish and Taina K. Korhonen and Richard A. Hull and Sheila I Hull},
  journal={Infection and immunity},
  volume={55 12},
The uropathogenic strain Escherichia coli IH11128 (O75:K5:H-) exhibits a mannose-resistant O75X adhesin. The genes encoding the O75X adhesin were cloned from a clinical strain and transferred to E. coli K-12 derivatives. The recombinant plasmids were found to express a 15-kilodalton fimbrial subunit protein, a fimbrialike extracellular structure, and mannose-resistant hemagglutination. An indirect immunofluorescence assay was used to study attachment of the clone and purified adhesin to frozen… CONTINUE READING