Molecular cloning of multiple cDNAs encoding human enzymes structurally related to 3α-hydroxysteroid dehydrogenase

@article{Qin1993MolecularCO,
  title={Molecular cloning of multiple cDNAs encoding human enzymes structurally related to 3$\alpha$-hydroxysteroid dehydrogenase},
  author={Ke-nan Qin and Maria I. New and K. -C. Cheng},
  journal={The Journal of Steroid Biochemistry and Molecular Biology},
  year={1993},
  volume={46},
  pages={673-679}
}
  • K. Qin, M. New, K. Cheng
  • Published 1993
  • Medicine, Biology
  • The Journal of Steroid Biochemistry and Molecular Biology
Rat liver 3 alpha-hydroxysteroid dehydrogenase cDNA was previously cloned by us. In this study, we used the rat cDNA as the probe to screen a human liver lambda gt11 cDNA library. A total of four different cDNAs were identified and sequenced. The sequence of one of the cDNAs is identical to that of the human chlordecone reductase cDNA except that our clone contains a much longer 5'-coding sequence than previously reported. The other three cDNAs display high degrees of sequence homology to those… Expand
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References

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TLDR
The protein expressed in bacteria was highly active in androsterone reduction in the presence of NAD as cofactor, and this activity was inhibited by indomethacin, a potent inhibitor of 3αHSD. Expand
Molecular cloning of rat liver 3α-hydroxysteroid dehydrogenase and identification of structurally related proteins from rat lung and kidney
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TLDR
The predicted amino acid sequence of 3α-hydroxysteroid d dehydrogenase was related to sequences of several other aldo-keto reductases such as bovine prostaglandin F synthase, human chlordecone reduct enzyme, human aldose reductase,human aldehyde reductasing and frog lens epsilon-crystallin, suggesting that these proteins belong to the same gene family. Expand
Cloning and sequencing of the cDNA for rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase.
TLDR
The sequence data presented suggests that 3 alpha-HSD, prostaglandin F synthase, and aldehyde/aldose reductases are members of a common gene family. Expand
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TLDR
To investigate the primary structure and expression of chlordecone reductase (CDR), a library of human liver cDNAs cloned in the expression vector lambda gt11 was screened and an 800 bp cDNA was isolated that directed synthesis of a fusion protein recognized by polyclonal anti-CDR antibodies. Expand
Full Length cDNA Structure and Deduced Amino Acid Sequence of Human β-Hydroxy-5-Ene Steroid Dehydrogenase
Polyclonal antibodies raised against 3β-hydroxysteroid dehydrogenase isolated from human placenta were used to screen a λgt11 expression cDNA library from the same tissue. The protein deduced fromExpand
Structural similarity of bovine lung prostaglandin F synthase to lens epsilon-crystallin of the European common frog.
TLDR
Comparison of the amino acid sequence of PGF synthase with the National Biomedical Research Foundation protein data base reveals that the result suggests that European common frog lens epsilon-crystallin is identical to bovine lung P GF synthase. Expand
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TLDR
Striking differences were found in the protein band patterns in kidney, lung, and testis, suggesting the existence of multiple antigenically related proteins in different tissues. Expand
Prostaglandin dehydrogenase activity of purified rat liver 3α-hydroxysteroid dehydrogenase
TLDR
Although the 3 α-HSD has many properties in common with the 9-hydroxyprostaglandin dehydrogenase of rat kidney, rat kidney contains no protein that is immunodetectable with polyclonal antibody raised against the purified 3α- HSD. Expand
Aldose reductase and ϱ‐crystallin belong to the same protein superfamily as aldehyde reductase
TLDR
The first primary sequence data for the rat lens enzyme is presented, obtained by amino acid and cDNA analysis, and it is proposed that aldose reductase, aldehyde reduct enzyme and ϱ‐crystallin are members of a superfamily of related proteins. Expand
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Publisher Summary This chapter describes methods for the isolation of eukaryotic and prokaryotic genes by screening Escherichia coil expression libraries with antibody probes, using the bacteriophageExpand
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