Molecular cloning of genes under control of the circadian clock in Neurospora.

@article{Loros1989MolecularCO,
  title={Molecular cloning of genes under control of the circadian clock in Neurospora.},
  author={Jennifer J. Loros and S A Denome and Jay C. Dunlap},
  journal={Science},
  year={1989},
  volume={243 4889},
  pages={385-8}
}
To investigate the regulation of messenger RNA abundance by circadian clocks, genomic and complementary DNA libraries were screened with complementary DNA probes enriched, by means of sequential rounds of subtractive hybridization, for sequences complementary to transcripts specific to either early morning or early evening cultures of Neurospora. Only two morning-specific genes were identified through this protocol. RNA blot analysis verified that the abundance of the transcripts arising from… CONTINUE READING

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