Molecular cloning of fused, a gene required for normal segmentation in the Drosophila melanogaster embryo.

@article{Mariol1987MolecularCO,
  title={Molecular cloning of fused, a gene required for normal segmentation in the Drosophila melanogaster embryo.},
  author={Marie Christine Mariol and Thomas Pr{\'e}at and Bernadette Limbourg-Bouchon},
  journal={Molecular and cellular biology},
  year={1987},
  volume={7 9},
  pages={3244-51}
}
Using the chromosomal walk technique, we isolated recombinant lambda bacteriophage and cosmid clones spanning 250 kilobases (kb) in the 17C-D region of the X chromosome of Drosophila melanogaster. This region was known to contain the segment polarity gene fused. Several lethal fused mutations were used to define more precisely the localization of this locus. Southern analysis of genomic DNA revealed that all of them were relatively large deficiencies, the smallest one being 40 kb long. None of… CONTINUE READING