Molecular cloning of full-length Dmrt1 cDNA of Reeves turtle (Chinemys reevesii).

@article{Hoshi2008MolecularCO,
  title={Molecular cloning of full-length Dmrt1 cDNA of Reeves turtle (Chinemys reevesii).},
  author={Hidenobu Hoshi and Aya Nakao},
  journal={The Journal of veterinary medical science},
  year={2008},
  volume={70 7},
  pages={687-92}
}
We cloned and sequenced a full-length open reading frame turtle dmrt1 cDNA (Crdmrt1) that was 1,504 bp in length and encoded 371 amino acid residues. RT-PCR analysis in different tissues of adult male turtle showed that the Crdmrt1 cDNA fragment was only detected in the testis. The amino acid sequence derived from Crdmrt1 demonstrated high homology to sequences from dmrt1 of Pelodiscus sinensis (92% identities and 93% positives) and Elaphe quadrivirgata (75% identities and 83% positives). The… CONTINUE READING