Molecular cloning of feline interferon cDNA by direct expression.

  title={Molecular cloning of feline interferon cDNA by direct expression.},
  author={Nobuyuki Nakamura and T Sudo and Satoru Matsuda and Amiel Yanai},
  journal={Bioscience, biotechnology, and biochemistry},
  volume={56 2},
A cDNA sequence coding for feline interferon has been cloned for the first time by screening a cDNA library constructed using Okayama-Berg vector and mRNA derived from the feline cells (LSA-I) induced by TPA (12-o-tetradecanoylphorbor 13-acetate) for the ability of transient expression to produce feline interferon in COS1 monkey cells. The amino acid sequence, deduced from the nucleotide sequence by comparing it with the sequences of other mammalian IFNs, consists of 171 amino acids with 6… CONTINUE READING


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