Molecular cloning of enantioselective ester hydrolase from Bacillus pumilus DBRL-191.

Abstract

A gene from Bacillus pumilus expressed under its native promoter was cloned in Escherichia coli. Recombinant B. pumilus esterase (BPE) affects the kinetic resolution of racemic mixtures such as unsubstituted and substituted 1-(phenyl)ethanols (E approximately 33-103), ethyl 3-hydroxy-3-phenylpropanoate (E approximately 45-71), trans-4-fluorophenyl-3… (More)

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@article{Rasool2005MolecularCO, title={Molecular cloning of enantioselective ester hydrolase from Bacillus pumilus DBRL-191.}, author={S. Rasool and Sarojini Johri and Syed Riyaz-ul-Hassan and Q. Maqbool and Vijeshwar Verma and Surrinder Koul and Subhash Chandra Taneja and Ghulam Nabi Qazi}, journal={FEMS microbiology letters}, year={2005}, volume={249 1}, pages={113-20} }