Molecular cloning of cone opsin genes and their expression in the retina of a smelt, Ayu (Plecoglossus altivelis, Teleostei).

@article{Minamoto2005MolecularCO,
  title={Molecular cloning of cone opsin genes and their expression in the retina of a smelt, Ayu (Plecoglossus altivelis, Teleostei).},
  author={Toshifumi Minamoto and Isamu Shimizu},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={2005},
  volume={140 2},
  pages={197-205}
}
Five cone opsin genes of landlocked ayu fish (Plecoglossus altivelis) were cloned, and the expression patterns of these genes were investigated. AYU-LWS, -RH2-1, -RH2-2, -SWS1-1, and -SWS1-2 were isolated and had high (more than 75%) identity with red, green, green, UV, and UV-sensitive opsin, respectively, genes of other fish reported previously. The results of Southern blotting experiments showed that each gene is present as a single copy. Gene expression was measured by RT-PCR using four… CONTINUE READING

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