Molecular cloning of cDNA encoding Sm autoantigen: derivation of a cDNA for a B polypeptide of the U series of small nuclear ribonucleoprotein particles.

@article{Ohosone1989MolecularCO,
  title={Molecular cloning of cDNA encoding Sm autoantigen: derivation of a cDNA for a B polypeptide of the U series of small nuclear ribonucleoprotein particles.},
  author={Yasuo Ohosone and Tsuneyo Mimori and Andrew J. Griffith and Masashi Akizuki and Masato Homma and Joseph F Craft and John A. Hardin},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 11},
  pages={4249-53}
}
The Sm snRNPs play a central role in the processing of pre-mRNA. Anti-Sm antibodies, the diagnostic hallmark of systemic lupus erythematosus, target the B'/B and D polypeptides of these snRNPs. We have used patient autoantibodies to clone a cDNA from a human fibroblast cDNA library that encodes the full length of a polypeptide identical with, or closely related to, polypeptide B. This cDNA is comprised of 1139 bases and contains an open reading frame of 855 nucleotides that is capable of… CONTINUE READING