Molecular cloning of an immunogenic and acid-induced isocitrate dehydrogenase gene from Coxiella burnetii.

@article{Nguyen1999MolecularCO,
  title={Molecular cloning of an immunogenic and acid-induced isocitrate dehydrogenase gene from Coxiella burnetii.},
  author={S Van Nguyen and Ho To and Tsuyoshi Yamaguchi and Hideto Fukushi and Katsuya Hirai},
  journal={FEMS microbiology letters},
  year={1999},
  volume={175 1},
  pages={101-6}
}
The Coxiella burnetii icd gene encoding an immunogenic dimeric NADP(+)-dependent isocitrate dehydrogenase (IDH) was cloned by screening a C. burnetii genomic library with a human positive serum and sequenced. The predicted gene product consists of 427 amino acids (M(r) = 46,600) and showed high identity to the IDHs of Escherichia coli (74%), Salmonella enterica (73%) and IDH-I of Vibrio sp. (71%). The cloned gene complemented an icd-defective E. coli mutant producing a recombinant IDH that had… CONTINUE READING