Molecular cloning of an apoptosis-inducing protein, pierisin, from cabbage butterfly: possible involvement of ADP-ribosylation in its activity.

@article{Watanabe1999MolecularCO,
  title={Molecular cloning of an apoptosis-inducing protein, pierisin, from cabbage butterfly: possible involvement of ADP-ribosylation in its activity.},
  author={Masanori Watanabe and Tomohiro Kono and Yuko Matsushima-Hibiya and Takashi Kanazawa and Nobuyasu Nishisaka and Tadamitsu Kishimoto and Kotaro Koyama and Takashi Sugimura and Keiji Wakabayashi},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1999},
  volume={96 19},
  pages={10608-13}
}
We have previously reported that the cabbage butterfly, Pieris rapae, contains a 98-kDa protein, named pierisin, that induces apoptosis in a variety of human cancer cell lines. In the present study, sequencing and cloning of a cDNA encoding pierisin was accomplished. PCR-direct sequencing showed that the gene encodes an 850-amino acid protein with a calculated molecular weight of 98,081. An intact clone at the amino acid level encompassing the entire coding region was obtained by recombination… CONTINUE READING

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Watanabe et al

  • Natori, S. Res. Immunol. 141, 938–939. Biochemistry
  • Proc. Natl. Acad. Sci. USA 96
  • 1999

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