Molecular cloning of a recA-like gene of Methylophilus methylotrophus and identification of its product.

Abstract

A recombinant plasmid, pPF5, has been constructed which contains a 5.5-kb fragment of Methylophilus methylotrophus DNA inserted into pAT153, and which confers resistance to UV and mitomycin C (MC) upon Escherichia coli recA mutants. Hybridisation analysis indicates that sequence homology exists between the cloned DNA and a fragment of E. coli chromosomal… (More)

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