Molecular cloning of a novel importin alpha homologue from rice, by which constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-protein is preferentially nuclear imported.

@article{Jiang2001MolecularCO,
  title={Molecular cloning of a novel importin alpha homologue from rice, by which constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-protein is preferentially nuclear imported.},
  author={Chang-Jie Jiang and Kazuaki Shoji and Rikyu Matsuki and A. Hakan Baba and Naoyuki Inagaki and Hiroshi Ban and Toshio Iwasaki and Naoko Imamoto and Yoshihiro Yoneda and Xing Wang Deng and Noriaki Yamamoto},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 12},
  pages={9322-9}
}
Nuclear import of proteins that contain classical nuclear localization signals (NLS) is initiated by importin alpha, a protein that recognizes and binds to the NLS in the cytoplasm. In this paper, we have cloned a cDNA for a novel importin alpha homologue from rice which is in addition to our previously isolated rice importin alpha1a and alpha2, and we have named it rice importin alpha1b. In vitro binding and nuclear import assays using recombinant importin alpha1b protein demonstrate that rice… CONTINUE READING