Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates.

@article{Tang1996MolecularCO,
  title={Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates.},
  author={Wen Lu Tang and Michaeline Bunting and Guy A. Zimmerman and Thomas M McIntyre and Stephen M. Prescott},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 17},
  pages={10237-41}
}
Diacylglycerol (DAG) is a second messenger that activates protein kinase C and also occupies a central role in phospholipid biosynthesis. Conversion of DAG to phosphatidic acid by DAG kinase regulates the amount of DAG and the route it takes. We used degenerate primers to amplify polymerase chain reaction products from cDNA derived from human endothelial cells. A product with a novel sequence was identified and used to clone a 2.6-kilobase cDNA from an endothelial cell library. When transfected… CONTINUE READING
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