Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjögren's syndrome.

  title={Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sj{\"o}gren's syndrome.},
  author={K. Griffith and E. K. Chan and C. Lung and J. C. Hamel and X. Guo and K. Miyachi and M. Fritzler},
  journal={Arthritis and rheumatism},
  volume={40 9},
  • K. Griffith, E. K. Chan, +4 authors M. Fritzler
  • Published 1997
  • Biology, Medicine
  • Arthritis and rheumatism
  • OBJECTIVE To identify a Golgi complex autoantigen bound by Sjögren's syndrome (SS) autoantibodies. METHODS Serum from a patient with secondary SS and anti-Golgi antibodies was used as a probe to isolate a complementary DNA (cDNA) insert from a HeLa cDNA library. RESULTS A 3.7-kb cDNA encoding a 56-kd recombinant protein was immunoprecipitated by the human anti-Golgi serum and immune rabbit serum. Western blot analysis showed that the immune rabbit sera recognized a protein of 97 kd (golgin… CONTINUE READING
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