Molecular cloning of a novel 120-kDa TBP-interacting protein.

  title={Molecular cloning of a novel 120-kDa TBP-interacting protein.},
  author={Satomi Yogosawa and Yuko Makino and Takeshi Yoshida and Takeo Kishimoto and Masaaki Muramatsu and Tomonari Tamura},
  journal={Biochemical and biophysical research communications},
  volume={229 2},
TATA-binding protein (TBP) is a central component for transcriptional regulation and is a target for various transcription regulators. Using histidine-tagged TBP as a ligand for affinity-purification of proteins bound to TBP, we purified a 120-kD protein, termed TBP-interacting protein 120 (TIP120), from rat liver nuclear extracts. The entire cDNA sequence of TIP120 contained an open reading frame encoding a novel polypeptide of 1230 amino acids. The recombinant TIP120 interacted directly with… CONTINUE READING