Molecular cloning of a gene required for DNA replication in Ustilago maydis

@article{Onel1997MolecularCO,
  title={Molecular cloning of a gene required for DNA replication in Ustilago maydis},
  author={Kenan Onel and William K Holloman},
  journal={Molecular and General Genetics MGG},
  year={1997},
  volume={253},
  pages={463-468}
}
TSD2, a gene necessary for DNA synthesis in Ustilago maydis, was cloned by complementation of the temperature sensitive growth defect of a mutant known previously as pol1-1 and renamed here tsd2-1. Linkage analysis established that the cloned fragment contained an allele of tsd2-1 and not a suppressor. DNA sequence determination of the cloned DNA fragment indicated the presence of a single large uninterrupted open reading frame capable of encoding a protein of 845 amino acids without homology… CONTINUE READING

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