Molecular cloning of a gene required for DNA replication in Ustilago maydis

  title={Molecular cloning of a gene required for DNA replication in Ustilago maydis},
  author={Kenan Onel and William K Holloman},
  journal={Molecular and General Genetics MGG},
TSD2, a gene necessary for DNA synthesis in Ustilago maydis, was cloned by complementation of the temperature sensitive growth defect of a mutant known previously as pol1-1 and renamed here tsd2-1. Linkage analysis established that the cloned fragment contained an allele of tsd2-1 and not a suppressor. DNA sequence determination of the cloned DNA fragment indicated the presence of a single large uninterrupted open reading frame capable of encoding a protein of 845 amino acids without homology… CONTINUE READING


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