Molecular cloning of a cDNA coding for a region of an apoprotein from the 'insoluble' mucin complex of rat small intestine.

@article{Hansson1994MolecularCO,
  title={Molecular cloning of a cDNA coding for a region of an apoprotein from the 'insoluble' mucin complex of rat small intestine.},
  author={Gunnar C Hansson and Dan Baeckstr{\"o}m and Ingemar Carlstedt and Karin Klinga-Levan},
  journal={Biochemical and biophysical research communications},
  year={1994},
  volume={198 1},
  pages={181-90}
}
The major part of rat small intestinal mucins occurs as an 'insoluble' glycoprotein complex unextractable in 6 M guanidinium chloride unless disulfide bonds are cleaved. One of the trypsin-resistant high-glycosylated domains of this complex (glycopeptide A) was recently isolated. We have now deglycosylated it with HF, injected it into rabbits and the obtained antiserum was used for expression cloning providing a cDNA clone (VR-1A). This clone contained an open reading frame of 235 amino acids… CONTINUE READING

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