Molecular cloning of N-methylputrescine oxidase from tobacco.

  title={Molecular cloning of N-methylputrescine oxidase from tobacco.},
  author={Akira Katoh and Tsubasa Shoji and Takashi Hashimoto},
  journal={Plant & cell physiology},
  volume={48 3},
Nicotine biosynthesis in Nicotiana species requires an oxidative deamination of N-methylputrescine, catalyzed by N-methylputrescine oxidase (MPO). In a screen for tobacco genes that were down-regulated in a tobacco mutant with altered regulation of nicotine biosynthesis, we identified two homologous MPO cDNAs which encode diamine oxidases of a particular subclass. Tobacco MPO genes were expressed specifically in the root, and up-regulated by jasmonate treatment. Recombinant MPO protein… CONTINUE READING