Molecular cloning of HEK, the gene encoding a receptor tyrosine kinase expressed by human lymphoid tumor cell lines.

@article{Wicks1992MolecularCO,
  title={Molecular cloning of HEK, the gene encoding a receptor tyrosine kinase expressed by human lymphoid tumor cell lines.},
  author={Ian P Wicks and Dave Wilkinson and Evelyn J Salvaris and Andrew W. Boyd},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 5},
  pages={1611-5}
}
We describe the molecular cloning of a receptor tyrosine kinase from a cell line (LK63) derived from a case of human pre-B-cell leukemia. We have previously shown that a monoclonal antibody (IIIA4) raised against LK63 recognized a glycosylated, cell-surface 135-kDa molecule (HEK), which displayed tyrosine kinase activity in vitro. The HEK protein was purified by using a IIIA4 antibody column and both N-terminal and internal amino acid sequences were obtained. A 51-mer degenerate oligonucleotide… CONTINUE READING

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