Molecular cloning of Ebitein1: a novel extracellular signal-regulated kinase 2-binding protein in testis.

@article{Miura2008MolecularCO,
  title={Molecular cloning of Ebitein1: a novel extracellular signal-regulated kinase 2-binding protein in testis.},
  author={Kenji Miura and Junko Imaki},
  journal={Biochemical and biophysical research communications},
  year={2008},
  volume={368 2},
  pages={
          336-42
        }
}
  • K. Miura, J. Imaki
  • Published 4 April 2008
  • Biology, Medicine
  • Biochemical and biophysical research communications
We cloned a cDNA encoding a novel extracellular signal-regulated kinase (ERK) 2-binding protein, EBITEIN1, by yeast two-hybrid screening. Northern and Western blotting experiments showed that the transcript and protein were expressed in the testes. Furthermore, immunohistochemical experiments showed that EBITEIN1 existed at high levels in round spermatids, but at very low levels or not at all in other testicular cells. During spermatogenesis, EBITEIN1 was first translated after meiosis when… Expand
Identification of ERK2-binding domain of EBITEIN1, a novel ERK2-binding protein.
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Two domains are proposed in EBITEIN1: one is the minimal sequence for binding ERK2 (EB domain) and the other is the EBiteIN1 C-terminal domain (ECT domain) which might pave the way for further empirical and bioinformatic analyses of EBITEin1- and ERK 2-mediated events. Expand
ERK2-binding domain is required for phosphorylation of EBITEIN1, a potential downstream interactor of ERK2.
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  • Biology, Medicine
  • Biochemical and biophysical research communications
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